In our class we have been referring to them as macromolecules since they are necessary for life. Visit the oklahoma community college website biology labs onlines organic molecules website. Instead of verifying the macromolecules found in food, students use their knowledge of the macromolecules in food to determine the characteristics of specific biological indicators. Macromolecule virtual lab hazleton area high school.
When we eat food, we take in the large biological molecules found in the food, including carbohydrates, proteins, lipids such as fats, and nucleic acids such as dna, and use them to power our cells and build our bodies. The basis for this test is that starch is a coiled polymer of. Macromolecules i have developed an effective lesson that uses printable macromolecule boxes that my students really enjoy using. The more the whites spread out the older the egg is. What types of foods supply carbohydrates, proteins and. If you enjoy crackers, opt for ones that are made with 100% whole grains and seeds. Testing for the macromolecules occurred by using specific reagents on each macromolecule. The biurets test identifies proteins by oxidizing 46 peptide bonds of a protein using. Common foods, which often consist of plant materials or substances derived from.
Honey 304 predicted macromolecule starch starch protein glucose test to be used. The tests will tell if the macromolecule is present, but not in what amount. In addition to animalbased seafood options, there are also plantbased foods that contain nucleic acids. In the first post, i talked about a demonstration i use with my science class to show what digestion looks and feels. Present students with a master mix of all the food sources so that they have a positive reaction to all three indicators. The starch test was used for the presence of starch. Common foods, which often consist of plant materials or. The macromolecules are assembled by joining several smaller units. Sep 15, 2008 which macromolecules should be present in the following food substances. Break the saltine cracker in half do not eat the entire cracker at once. Mcmush lab 5 mcmush lab testing for the presence of macromolecules objective students will learn confirmation tests for the presence of glucose, starch, lipids and proteins.
Feb 19, 2008 a plain dry cracker turns black in the presence of iodine. The purpose of this lab was to determine the different types of nutrients in each of the unknown solutions, aj. What the functions of each macromolecule are in cells. Identifying macromolecules in common foods essay 2217 words. Tis the season for onebite party snacksbut which cracker to place them atop, ritz or triscuit. Testing for simple sugars with benedicts solution youtube. The cracker test is a way for people to measure how many carbohydrates to consume. Instead of verifying the macromolecules found in food, students use their knowledge of the macromolecules in food to determine the characteristics of specific.
After being exposed to saliva, the cracker now shows a positive test in the presence of benedicts solution and heat. According to the gordon research institute, sardines have the highest levels of nucleic acids, which typically comprise 1. Rinse out your test tube and record your results for the starch test in data table 1. Answer the following questions based on your reading. You will be testing 5 different foods, one of which will be an unknown. Click the button to the left of the single best answer. Dec 23, 20 which cracker is better, ritz or triscuit. Your vote could end the ceaseless cracker conflict. Once there carefully read and follow instructions to test substances for the presence of the four macromolecules.
For simple sugars, the benedicts solutions was used as the reagent. Do not chew the cracker, simply allow your saliva enzymes to break down the cracker. Benedicts solution is used to test for simple sugar. Wheat flour may refer to processed white flour or wholegrain wheat flour. Macromolecule lab with potatoes in biology, a few days ago, we conducted a lab about macromolecules. How the scientific method was used in medicine man install run mac os x mountain lion 10. Testingfor0macromolecules0 thisisthesametestyouwillperformfortheingredien tsinyoursaladdressing. Compare and contrast the difference between monosaccharides and disacharides. Oct 20, 20 as an egg ages the whites of the egg will become thinner. Hydrogenation causes the fat to harden more, which can then be used to make crispy foods such as potato chips, crackers, and cookies, as well as some types of salad dressings. This activity was created by a quia web subscriber. Define monosaccharide, disaccharide, and polysaccharide, and give.
Use benedicts solution to test for simple sugars, such as fructose or glucose. Simple carbohydrates can form either a single ring structure monosaccharides or a double ring structure disaccharides formed when a pair of monosaccharides bond. The gardening channel with james prigioni recommended for you. How a 30second cracker test reveals how much carbs you can. Background the most common macromolecules organic compounds found in living organisms are lipids, carbohydrates, proteins, and nucleic acids. Macromolecules are large molecules that are essential to the structure and function of the cell. Identifying macromolecules lab purpose to test for the presence of macromolecules in various materials. It is high in saturated fat, like cheese and other dairy products, but eaten as part of a healthy, mediterraneanstyle diet, yogurt provides lots of probiotics that can help with digestion and possibly heart disease and obesity. Compare the test results of the snack taken from the scene of the crime to the residues found. Add 10 drops of benedicts solution to each test tube. The center for elearning multimedia team motion graphic eduardo rodriguez filming eduardo rodriguez, david cheng photography eduardo rodriguez video edit eduardo rodriguez audio.
Cut a 4 x 4 inch square from the butcher paper for each sample. Freeman, quillin, allison, biological science fifth edition, pearson, 2014 heidemann, merle, gerald urquhart, and jaime jamison. A positive control will result in a color change indicating the presence of the compound you are testing for. Place the test tubes in the hotwater bath for 35 minutes. Muffins, crackers, cookies and cakes are refined grains.
However if you chew up the cracker and test it again, it does test positive. Testing food for biological macromoleculesnutrients by. Lipids are organic, meaning that they contain carbon atoms. It is also used to test for the presence of glucose in urine. Compare the results of the mcmush to the negative and positive controls to determine if there is protein present. Some sugars such as glucose are called reducing sugars because they are capable of transferring hydrogens electrons to other compounds, a process called reduction. Biologically important molecules post lab biology homework help.
In contrast to whole grains, these foods are devoid of fiber and low in vitamins. The ph indicators detection is based upon observing a chemical change that takes place most often a change in color. You may reset all the answers by pressing the reset button. Highmoisture baked goods such as pumpkin bread while safe at room temperature for a few days should not be mailed because they will most likely mold before.
As an egg ages the whites of the egg will become thinner. Dive into the different types of macromolecules, what they are made up. This lab will test various food sources for various types of compounds. A test for reducing sugars carbohydrates are divided into two groups based on the complexity of their structure. I am testing for the presence of starch, which pair would indicate a positive test. A package of peanut butter crackers contains 7g of fat, 16g of carbohydrates and 4g of protein. Food substance predicted macromolecule test to be used saltine crackers. A benedicts test is used to determine the presence of reducing sugars such as fructose, glucose, maltose and lactose.
Answering the questions below will help you practice using what you know about macromolecules. Fill each test tube with 3 ml of one substance as indicated above. Mar 02, 2015 so i did this same assignment with my class. Trace the path that the nutrients in a cheeseburger. There are 3 different gamesactivities that i use to help my students grasp this concept. About how many typical amino acids are found in proteins. The introductory lecture on enzyme function requires at least 25 minutes of class time. Component carbohydrate sugar fat protein happy meal the organic tests performed are for the presence of starch, simple sugars, proteins and fat. Eggs are graded for freshness by observing the whites. Frequently asked questions national agricultural library. Macromolecule note sheet this note sheet corresponds the the powerpoint that goes into detail about macromolecules.
Testing food for biological macromolecules biology lab report tweet. Lamothe think that the suspects might still have food residues in their hands. The amount of energy that can be tapped is related to the structure you observed in activity 2. Bland or mild crackers are sometimes used as a palate cleanser in food product testing or flavor testing, between samples. Human digestion demonstrationthe bread in the bag demo. Benedicts solution doesnt identify the specific sugar in a sample, but the color produced by the test can indicate whether a small or large quantity of sugar is present. Sep 23, 2009 identifying macromolecules in food lab adapted from. Customize when and where you study by printing the worksheet. This activity will put your amylase enzymes to the test. Students will then apply the test procedures to a slurry made from a mcdonalds happy meal to determine the presence of these macromolecules in the sample. Is there a macromolecule that the tests in this lab did not test for. The intent of this article is to describe the conversion of this standard cookbook lab into an inquiry investigation.
Biology identifying macromolecules purpose to test for the presence of macromolecules in various foods. A cracker is a flat, dry baked food typically made with flour. We did so by conducting a series of tests on each solution. How are indicators used to test for the presence of organic compounds. The student reactions are even better than with crackers and as i said before. We also acknowledge previous national science foundation support under grant numbers 1246120. Identification of macromolecules in food is a standard introductory high school biology lab. The modern cracker is somewhat similar to nautical ships biscuits, military hardtack, chacknels, and sacramental bread. Flavorings or seasonings, such as salt, herbs, seeds, or cheese, may be added to the dough or sprinkled on top before baking. What types of foods supply carbohydrates, proteins and lipids. Biomolecules in my food pottsgrove school district. If you can tolerate dairy, fullfat yogurt is a source of several nutrients. You may want to make one big chart with room for this information as well as for simple sketches. An unknown food item is tested using iodine, biuret, sudan, benedicts and paper.
These macromolecules are large carbon based structures. Somebody entered the school cafeteria and stole all the snacks, the cafeteria lady called for the 10th graders help since they are macromolecule experts. If a color change occurred, then the sample would prove to be positive for that macromolecule. For example, if you are testing for the presence of protein in an unknown. By using each of the four indicators benedicts, iodinepotassium iodide, biuret, and paper towels on these four foods glucose, cornstarch, egg albumin, and vegetable oil, we were able to understand how particular solutions reacted with the indicators if they did or did not contain that particular macromolecule. Which contains more protein cn bonds, egg albumen or honey. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Which of the following best describes the formation of a macromolecule. Both of these activities could be done on the same day as the teachers macromolecule lesson. Instead of looking for either low or highcarb foods when choosing carbohydrates, pick healthy carbs over unhealthy carbs. Each macromolecule test consisted of five test tubes of the food item individually diluted into solutions for reacting each with benedicts reagent to show the presence of sugar, ik2i for starch presence, and buirets reagent for protein presence. The preferred macromolecule to use as an energy source is a carbohydrate.
Methods the tests performed were iodine testing for starch, benedicts test for glucose, the grease spot test for lipids, and the cuso4s test for protein. What has the saliva done to the cracker to cause this result. Mcmush lab 5 mcmush lab testing for the presence of macromolecules. When testing the crackers, both the protein and lipids test had a negative reaction, because in the lipid test there was not a translucent spot the mixture of crushed up crackers and water had evaporated, and in the protein test the color did not change. Click on the scroll down bar on the right hand side and go to starches. A simple paper test was used evaluting lipid existence for each food. Carbohydrates in food can take the form of sugars, starches, and fiber.
In this experiment, the testing was to notice if there were any macromolecules. Macromolecules inquiry the american biology teacher. Environmental education resources to commemorate earth days 50th anniversary. In this lab we were given a food, either egg white, egg yolk, potato, apple, onion, strawberry or lemon.
Soda crackers instant potatoes 10% sucrose solution chicken tuna pasta rice jjjstudent experimental examples problem 1 students are to determine the characteristics of a positive reaction. Using saliva in the mouth, the test involves the use of an. One characteristic of life is that living things are made up of molecules containing carbon. A lipid is a fatlike molecule and is a major building block of the cells of animals.
Snack foods crackers, chips, cheese doodles, or similar foods, crushed. Dive into the different types of macromolecules, what they are made up of, and how. Madison southern high school biology slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. The following day you could address a second macromolecule group, and so on. Be sure to mash your food into a paste before testing. Testing food for biological macromolecules biology lab report. Test exactly what you know about macromolecules by completing this interactive quiz.
When you test a cracker for sugar, it does not test positive. Simple carbohydrates include familiar sugars such the. If this lab is going to be done over a series of days, you will need to refrigerate the gelatin. Macromolecule testing introduction organic molecules.
Human digestion demonstration the bread in the bag. How to build a hinged hoophouse for a raised bed garden duration. Obtain 8 clean test tubes and place them in a test tube rack. Flour packages usually make it obvious with terms such as allpurpose and enriched that indicate refined white flour, or whole wheat, and 100 percent whole grain, which signifies a. Heat source microwave, hot plate, hot water bath, or heating pad.
Although saltine crackers are a popular snack, they are low in nutrients and high in starch. How are the reagents useful in determining which macromolecules are present. A list of testing laboratories accredited by the fsis. What is one big thing you learned from performing this lab. In this experiment, biuret will be the indicator for proteins. Before testing our substance, we tested substances that we knew contained protein, starch, glucose and lipids. Even crackers with no added sugar may still test positive for glucose. Carbohydrates, fats, and proteins each provide varying amounts of energy for the body. To perform tests that detect the presence of carbohydrates, lipids, proteins, and.
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